Plaque assay and cloning of scrub typhus rickettsiae in irradiated L-929 cells.
نویسندگان
چکیده
It was demonstrated that gamma-irradiated L-929 cells support plaque formation by three strains of Rickettsia tsutsugamushi and representative species of the spotted fever and typhus group rickettsiae. Sensitivity of the plaque assay for detection of viable scrub typhus rickettsiae was similar to that achieved with intraperitoneal inoculation of random-bred mice. The concentration of irradiated cells and the temperature and length of incubation were all found to affect plaque size. A technique combining terminal dilution and plaque purification was used to obtain clones of three strains of scrub typhus rickettsiae.
منابع مشابه
Fever and Scrub Typhus Rickettsiae
The rickettsiae have always been difficult to study because convenient titration and isolation techniques have not been available for the study of these organisms. In recent reports (3, 5), however, we demonstrated the use of a sensitive plaque test for measuring concentrations of typhus and spotted fever rickettsiae. Studies were then initiated to determine whether this assay system is appropr...
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 6 1 شماره
صفحات -
تاریخ انتشار 1977